Enzymatic fabrication of DNA nanostructures: extension of a self-assembled oligonucleotide monolayer on gold arrays.

TitleEnzymatic fabrication of DNA nanostructures: extension of a self-assembled oligonucleotide monolayer on gold arrays.
Publication TypeJournal Article
Year of Publication2005
AuthorsDC Chow, W-K Lee, S Zauscher, and A Chilkoti
JournalJournal of the American Chemical Society
Volume127
Issue41
Start Page14122
Pagination14122 - 14123
Date Published10/2005
Abstract

Nucleic acid nanostructures are useful as templates for bionanofabrication of composite molecular nanostructures in materials science, molecular electronics, and biosensing. Here, we demonstrate that terminal deoxynucleotidyl transferase, which repetitively adds mononucleotides to the 3' end of a short DNA initiator, can be used to rapidly fabricate DNA nanostructures up to 121 nm high with lateral dimensions from 0.1 to 4 mum in 2 h. These programmable scaffolds can potentially be employed to build more complex nanostructures consisting of natural or unnatural nucleotides with selective docking sites along the single-stranded DNA.

DOI10.1021/ja052491z
Short TitleJournal of the American Chemical Society