Molecular Recognition-Mediated Fabrication of Protein Nanostructures by Dip-Pen Lithography

TitleMolecular Recognition-Mediated Fabrication of Protein Nanostructures by Dip-Pen Lithography
Publication TypeJournal Article
Year of Publication2002
AuthorsJ Hyun, SJ Ahn, WK Lee, A Chilkoti, and S Zauscher
JournalNano Letters
Volume2
Issue11
Start Page1203
Pagination1203 - 1207
Date Published11/2002
Abstract

We describe the molecular recognition-mediated, stepwise fabrication of patterned protein nanostructures with feature sizes on the order of 200 nm. First, a self-assembled monolayer (SAM) of 16-mercaptohexadecanoic acid (MHA) is patterned onto gold by dip-pen nanolithography (DPN), and the unpatterned regions are passivated with a protein-resistant oligoethylene glycol-terminated alkanethiol SAM. Next, an amine-terminated biotin derivative is covalently conjugated with the chemically activated MHA SAM nanopattern. The surface is then incubated with streptavidin to form streptavidin nanostructures, mediated by molecular recognition between biotin and streptavidin. Finally, protein nanopatterns are fabricated by molecular recognition-mediated immobilization of biotinylated protein from solution. Our fabrication methodology is generically applicable because of the ubiquity of biotin-tagged molecules.

DOI10.1021/nl0257364
Short TitleNano Letters