Peptide-PEG amphiphiles as cytophobic coatings for mammalian and bacterial cells.

TitlePeptide-PEG amphiphiles as cytophobic coatings for mammalian and bacterial cells.
Publication TypeJournal Article
Year of Publication2006
AuthorsDJ Kenan, EB Walsh,, GA O'Toole, EG Carruthers, WK Lee, S Zauscher, CAH Prata, and MW Grinstaff
JournalChemistry & Biology
Volume13
Issue7
Start Page695
Pagination695 - 700
Date Published07/2006
Abstract

Amphiphilic macromolecules containing a polystyrene-adherent peptide domain and a cell-repellent poly(ethylene glycol) domain were designed, synthesized, and evaluated as a cytophobic surface coating. Such cytophobic, or cell-repellent, coatings are of interest for varied medical and biotechnological applications. The composition of the polystyrene binding peptide domain was identified using an M13 phage display library. ELISA and atomic force spectroscopy were used to evaluate the binding affinity of the amphiphile peptide domain to polystyrene. When coated onto polystyrene, the amphiphile reduced cell adhesion of two distinct mammalian cell lines and pathogenic Staphylococcus aureus strains.

DOI10.1016/j.chembiol.2006.06.013
Short TitleChemistry & Biology