High-Molecular-Weight Polynucleotides by Transferase-Catalyzed Living Chain-Growth Polycondensation.

TitleHigh-Molecular-Weight Polynucleotides by Transferase-Catalyzed Living Chain-Growth Polycondensation.
Publication TypeJournal Article
Year of Publication2017
AuthorsTang, L, Navarro, LA, Chilkoti, A, and Zauscher, S
JournalAngewandte Chemie International Edition
Volume56
Issue24
Start Page6778
Pagination6778 - 6782
Date Published06/2017
Abstract

We present terminal deoxynucleotidyl transferase-catalyzed enzymatic polymerization (TcEP) for the template-free synthesis of high-molecular-weight, single-stranded DNA (ssDNA) and demonstrate that it proceeds by a living chain-growth polycondensation mechanism. We show that the molecular weight of the reaction products is nearly monodisperse, and can be manipulated by the feed ratio of nucleotide (monomer) to oligonucleotide (initiator), as typically observed for living polymerization reactions. Understanding the synthesis mechanism and the reaction kinetics enables the rational, template-free synthesis of ssDNA that can be used for a range of biomedical and nanotechnology applications.

DOI10.1002/anie.201700991
Short TitleAngewandte Chemie International Edition
High-Molecular-Weight Polynucleotides by Transferase-Catalyzed Living Chain-Growth Polycondensation